Journal: Micromachines
Article Title: A Novel PCR-Free Ultrasensitive GQD-Based Label-Free Electrochemical DNA Sensor for Sensitive and Rapid Detection of Francisella tularensis †
doi: 10.3390/mi15111308
Figure Lengend Snippet: ( a ) The amperometric measurement curves obtained for eight concentrations of F. tularensis DNA (dsDNA) concentration using 5000 ppm GQDs, 5000 ppm GQDs, and GQDs-ssDNA attachment. ( b ) The linear calibration curve of DNA assays (10 5 nM–0.1 nM) with a correlation coefficient of 0.9712. ( c ) Sensogram of eight concentrations of F. tularensis DNA (dsDNA). ( d ) The amperometric measurement curves obtained only between 10 nM–0.01 nM.
Article Snippet: Synthetic oligonucleotides designed for the identification of the pathogenic bacterium F. tularensis , including the sequences of capture probe DNA (ssDNA) and complementary linear target DNA (ssDNA), were purchased from TIB Molbiol (Berlin, Germany).
Techniques: Concentration Assay